Regulatory

Part:BBa_K950002:Design

Designed by: Jakob Matthes   Group: iGEM12_Tuebingen   (2012-07-29)

yeast anb1 promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promotor is the 400bp long upstream region of the anb1 gene.

Source

sequence and information from http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=anb1

References

  1. BALASUBRAMANIAN, B., LOWRY, C. V. & ZITOMER, R. S. 1993. The Rox1 repressor of the Saccharomyces cerevisiae hypoxic genes is a specific DNA-binding protein with a high-mobility-group motif. Mol Cell Biol, 13, 6071-8.
  2. LOWRY, C. V., CERDAN, M. E. & ZITOMER, R. S. 1990. A hypoxic consensus operator and a constitutive activation region regulate the ANB1 gene of Saccharomyces cerevisiae. Mol Cell Biol, 10, 5921-6.
  3. LOWRY, C. V. & ZITOMER, R. S. 1984. Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast. Proceedings of the National Academy of Sciences, 81, 6129-6133.
  4. LOWRY, C. V. & ZITOMER, R. S. 1988. ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae. Mol Cell Biol, 8, 4651-8.